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Using a disposable C-Chip hemacytometer for semen analysis allows for clear visualization of the sperm that simply wasn't possible before. C-Chip DHC-S02 is a phase type hemacytometer (using phase contrast microscopy). Light microscopy could also be used for sperm counting, magnification x 200~400.

• Blood analysis(Hematology): Blood cell counting
• Cell culture: Cell concentration measurement, Cell viability test
• Microbiology : Fungal spores counting

The DHC-S consists of 100 small squares in the large center square.  Each small square measures 0.1 x 0.1 mm, with a chamber depth of 0.02 mm.  Each small square has a total volume of 2.0 x 10^-4mm³ or 2.0 x 10^-7cm³.

 Count all the cells in the 100 small squares within the large center square.  The large center square has a surface area of 1.0 mm² and a chamber depth of 0.02 mm.   The large center square has 0.02 mm³.   As there are 1000mm³ per ml, the center square represents a volume of 0.00002ml, so that equates to 1/ 0.0002ml = 50,000 (50,000 = 1/ (100 squares x 2.0 x 10^-7cm³).   The volume factor is then 50,000.

Cells per ml = Number of cells in 100 small squares x dilution factor x 50,000

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